Species:

Universal

Alternative Name:

25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

Assay Type:

Competitive

unit:

ng/mL

Standard:

200 ng/mL

Sensitivity:

0.91 ng/mL

Detection Range:

3.13-200 ng/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma and Other biological fluids

Research:

Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to HVD3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.